X-CLARITY Tissue Clearing System: The X-CLARITY™ Tissue Clearing System (Logos Biosystems) automates the CLARITY method, using electrophoretic tissue clearing (ETC) to remove lipids from fixed tissue-hydrogel hybrids. The process involves fixing tissues (4% PFA), hydrogel embedding/polymerization (37°C for 3-4 hours), and ETC (typically 60V / 1.2A / 37°C). Key steps include using ETC solution at 37°C…
The RoboRocker is ideal for microfluidic 3D cell and organoid culturing. Program the speed, tilt angles and pause times. This allows gravity driven perfusion at the desired flow rate and duration in several culture plates simultaneously. Use the RoboRocker to generate shear flow, create pulsed flow as if from a beating heart, or even continuous,…
The X-CLARITY™ systems and reagents standardize, simplify, and accelerate each step of the tissue clearing process. With the CLARITY method, preserved tissues are embedded in a hydrogel matrix and lipids are actively extracted through electrophoresis to create a stable and optically transparent tissue-hydrogel hybrid that is chemically accessible for multiple rounds of antibody labeling and…
This electrophoresis system makes a several weeks long slow process to a day or just free hours! The X-CLARITY™ systems and reagents standardize, simplify, and accelerate each step of the tissue clearing process. With the CLARITY method, preserved tissues are embedded in a hydrogel matrix and lipids are actively extracted through electrophoresis to create a…
Tissue clearing enables examination of biological structures at subcellular resolution in three dimensions. It uncovered the spatial and temporal plasticity of multicellular kidney structures that occur during homeostatic stress. This article will review the recent development in tissue clearing protocols and how it facilitated the study of renal transport mechanisms and remodelling of the kidney.Tissue…
TIPS FOR TISSUE CLEARING SUCCESS: Sample Preparation: 1. Fixation will vary depending on the sample type, meaning fixation time will also vary. 2. Fixation will vary depending on the sample size. For example, small/thin tissue only requires immersion fixation, but for large tissue and organ tissue, perfusion fixation must be done before immersion. 3. It’s…
X-CLARITY Tissue Clearing Systems enable to image tissue block or entire organ to view the 3D images and save weeks sample processing time for more consistent reliable results. X-CLARITY™ Tissue Clearing System The new X-CLARITY™ Tissue Clearing System from the imaging specialists at Logos Biosystems provides an all-in-one solution for electrophoretic tissue clearing. Combining an…
DeepLabel™ Antibody Staining Kit LB-C33001 DeepLabel™ Antibody Staining Kit is a set of non-toxic, ready-to-use reagents optimized for use with clarified tissues for effective antibody penetration and site-specific binding. With DeepLabel™, macromolecular probes rapidly and efficiently penetrate thick, protein-dense tissues at lower antibody concentrations. DeepLabel™ facilitates homogenous antibody staining with 2.6 X greater signal-to-background than…