The X-CLARITY™ systems and reagents standardize, simplify, and accelerate each step of the tissue clearing process. With the CLARITY method, preserved tissues are embedded in a hydrogel matrix and lipids are actively extracted through electrophoresis to create a stable and optically transparent tissue-hydrogel hybrid that is chemically accessible for multiple rounds of antibody labeling and…
This electrophoresis system makes a several weeks long slow process to a day or just free hours! The X-CLARITY™ systems and reagents standardize, simplify, and accelerate each step of the tissue clearing process. With the CLARITY method, preserved tissues are embedded in a hydrogel matrix and lipids are actively extracted through electrophoresis to create a…
Tissue clearing enables examination of biological structures at subcellular resolution in three dimensions. It uncovered the spatial and temporal plasticity of multicellular kidney structures that occur during homeostatic stress. This article will review the recent development in tissue clearing protocols and how it facilitated the study of renal transport mechanisms and remodelling of the kidney.Tissue…
TIPS FOR TISSUE CLEARING SUCCESS: Sample Preparation: 1. Fixation will vary depending on the sample type, meaning fixation time will also vary. 2. Fixation will vary depending on the sample size. For example, small/thin tissue only requires immersion fixation, but for large tissue and organ tissue, perfusion fixation must be done before immersion. 3. It’s…
X-CLARITY Tissue Clearing Systems enable to image tissue block or entire organ to view the 3D images and save weeks sample processing time for more consistent reliable results. X-CLARITY™ Tissue Clearing System The new X-CLARITY™ Tissue Clearing System from the imaging specialists at Logos Biosystems provides an all-in-one solution for electrophoretic tissue clearing. Combining an…
X-CLARITY is a tissue clearing method that uses a hydrogel to replace the lipids in tissue, making it transparent and allowing for high-resolution imaging. The X-CLARITY method is relatively simple and can be used to clear a variety of tissues, including whole organs. The X-CLARITY method begins with the fixation of tissue in a standard…