Nuclei Counting with Luna FL cell counter
**protocol is provided by one Luna FL user lab at UC Davis** contact info@wisbiomed.com for any questions or request complete protocol with dye pack preparation protocol.
-
-
Free Online Tool for cell counting
Introduction OnCellCounter™ is a web-based cell counter tool that provides cell counting and cell viability analysis for user-uploaded images. Developed by Logos Biosystems, a company specializing in automated cell counters, OnCellCounter™ delivers accurate and reliable cell counting results. How to Use OnCellCounter™ Using OnCellCounter™ is easy and involves just three simple steps: Step 1: Image…
-
Factors to consider when selecting a cell counter –How to Choose the Right Automated Cell Counter for Your Lab Needs
2023-05-11 Factors to consider when selecting a cell counter Introduction: If you work in a laboratory, you know how important it is to have accurate and efficient cell counting capabilities. Manual cell counting can be time-consuming, error-prone, and tedious, making automated cell counters a popular choice for many researchers. But with so many options on…
-
Luna III Bright-Field Automated Cell Counter
Feature Machine Learning Optimized for Excellence Experience unparalleled accuracy for individual cell recognition in highly aggregated samples, thanks to our state-of-the-art algorithms. Advanced Autofocus Technology Fast and accurate autofocusing for improved efficiency. Improved autofocus capabilities enhance cell recognition and measurement, enabling better decisions in downstream applications and protocols. Optimal Accuracy via Reanalysis Obtain accurate results…
-
QTX suggested protocols
Alcaligenes faecalis Stain: QUANTOM™ Total Cell Staining Dye Counting parameters Protocol name: DEFAULT Min. fluorescent object size: 1 µm Max. fluorescent object size: 50 µm Roundness: 30 Declustering level: 7 Detection sensitivity: 3 Bacillus amyloliquefaciens Stain: QUANTOM™ Total Cell Staining Dye Counting parameters Min. fluorescent object size: 1 µm Max. fluorescent object size: 50 µm…
-
FX7 Counting Range
The LUNA-FX7™ Automated Cell Counter has well performed the counting in a wide range of concentrations on both the Brightfield and the Fluorescence Cell Counting modes. Expressly, evaluating the LOQ ranges of the typical cell size has confirmed the utmost concentrations from 1.00E+04 to 2.50E+07 cells/mL . The detection ability on a wide concentration range…
-
Luna FX7 Cell Counter Calibration
The LUNA-FX7™ is calibrated prior to shipping. Calibration only needs to be performed -1) after any software/firmware updates, and -2) after switching trypan blue or erythrosin B brands or formulations. To perform calibration: 1. Press DATE & CALIBRATION. 48 2. Press Start calibration. The Calibration Step 1 window will appear 3. Remove any counting slide…
-
Publication: Luna FL application
The microscopic examination of Giemsa-stained thin and/or thick blood films (Giemsa microscopy) is the standard method of malaria diagnosis. However, the results of the diagnosis significantly depend on the skills of clinical technicians. Furthermore, sample preparation and analysis are laborious and time-consuming. Therefore, in this study, we investigated if a commercially available fluorescent cell counter,…
-
Count Cell Nuclei Particles with Luna FL Automated Cell Counter
Accurate counts is critical for many applications for isolated nuclei samples. Especially to make sure no cell debris or intact cells are counted as nuclei. Luna FL has bright field and fluorescent counting module. Use fluorescent module with AO/PI dye will get accurate counts. Nuclei particles will be stained by PI as red dots, while…
-
Automated cell counting and viability measurement of Insect cells (SF9): Comparison of staining method
Staining Protocols 1. Mix:• 10 μL TB, 0.4%• 10 μL cell sample2. Load 10 μL of stained cells.3. Perform analysis using the LUNA-FX7™.*Default protocol Fluorescence staining 1. Mix:• 2 μL pre-mixed dyes or 1μL per individual dye• 18 μL cell sample2. Load 10 μL of stained cells.3. Perform analysis using the LUNA-FX7™.*Note: SF9 cells may…