Optimizing Lysing Protocol

Optimizing Lysing Protocol is critical for efficient homogenization and improve extraction efficiency. The following factors are important to quickly optimize a homogenization protocol:

-Ratio of sample: buffer: beads And leave sufficient airspace in the lysing tube. Overfill will result low homogenization efficiency, while dry grinding may increase the risk of breaking lysing tube caps.

-Inspect samples particles visually for any large pieces and under microscope for evenly distributed particle sizes. The ideal particles size should be at uM ranges.

-Save protocol and document the optimization process.

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