Next Advance Tissue Lysing Kits

While the term “most efficient” can be subjective and depend on the specific application (sample type, downstream analysis), here’s a breakdown of Next Advance lysing kits that are designed for effective lysis in Bullet Blenders, categorized by sample type: For Small, Soft to Medium Organ Tissue Samples:

• PINK Lysis Kits: These kits contain zirconium oxide beads, which are ideal for gently lysing softer tissues like the brain, liver, and kidney. They are suitable for buffer volumes up to 200 µL and sample sizes up to 100 mg. For Small, Tough Organ Tissue Samples:
• GREEN Lysis Kits: These kits contain stainless steel beads, which provide more power for homogenizing tougher tissues such as muscle. They are also suitable for buffer volumes up to 200 µL and sample sizes up to 100 mg. For Larger, Soft to Medium Organ Tissue Samples:
• RED Lysis Kits: These kits also contain zirconium oxide beads but are designed for larger samples (up to 300 mg) and larger buffer volumes (200 µL to 600 µL). For Larger, Tough Organ Tissue Samples:
• NAVY Lysis Kits: These kits contain a custom mix of stainless steel beads, offering extra homogenizing power for challenging samples like bone, tumors, and hard-shelled insects. They are suitable for larger samples (up to 300 mg) and larger buffer volumes (200 µL to 600 µL). For Bacterial Lysis:
• YELLOW Lysis Kits: These kits contain beads specifically selected for high-yield bacterial lysis. They are suitable for bacterial pellets with buffer volumes between 200 µL and 600 µL. Important Considerations for Efficiency:
• Sample Type: The most efficient kit will always be the one specifically designed for your sample’s characteristics (softness/toughness, size). Using the wrong type of beads can lead to incomplete lysis or degradation of the target molecules.
• Buffer Volume: Ensure you choose a kit appropriate for your desired buffer volume to optimize the bead-to-sample ratio for efficient lysis.
• Tube Type: Next Advance offers both GATOR snap-cap tubes and RINO screw-cap tubes. RINO tubes are more robust and have a screw cap with a rubber seal, providing extra protection against sample loss, especially for tougher homogenization. These are recommended for use with Bullet Blender Storm and Gold models.
• RNase/DNase Free: Depending on your downstream application (e.g., RNA or DNA extraction), choose kits that are certified RNase and DNase-free to prevent nucleic acid degradation. How to Choose the Most Efficient Kit:
• Identify your sample type: Is it soft tissue, tough tissue, bacteria, etc.?
• Determine your desired buffer volume: How much buffer do you need for your downstream application?
• Consider the tube type: Do you need the extra security of screw-cap tubes?
• Check for RNase/DNase-free requirements: Is it critical to maintain the integrity of RNA or DNA? By carefully considering these factors and matching them to the specifications of the Next Advance lysis kits (Pink, Green, Red, Navy, Yellow), you can select the most efficient option for your specific needs with the Bullet Blender.

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