Luna FX7 Cell Counter Calibration

The LUNA-FX7™ is calibrated prior to shipping.

Calibration only needs to be performed

-1) after any software/firmware updates, and

-2) after switching trypan blue or erythrosin B brands or formulations.

To perform calibration:

1. Press DATE & CALIBRATION. 48

2. Press Start calibration. The Calibration Step 1 window will appear

3. Remove any counting slide from slide port.

4. Press START.

5. The Calibration Step 2 window will appear.

6. Mix one part stain with an equal volume of distilled water, PBS, or plain medium. Put 10 μL of the diluted stain into Chamber A of a PhotonSlide™.

7. Insert the slide face up into the port, so that Chamber A is to the left.

8. Press START or press SKIP if only fluorescence calibration is needed.

9. The Calibration Step 3 window will appear upon completion.

10. Load 10 μL of the LUNA-FX™ GF Calibration Beads into chamber A and 10 μL of the LUNA-FX™ RF Calibration Beads into chamber B of a new PhotonSlide™. 11. Wait for 30 seconds for the beads to settle down.

12. Insert the slide face up and chamber A first into the slide port.

13. Press START; or, press SKIP, if fluorescence calibration is not needed.

14. Upon completion, the calibration value(s) and calibration date will be updated.