Luna III for PBMC counting

The integration of a focal plane offset and advanced cell detection algorithm in the LUNA-III™ has significantly improved the accuracy and precision of PBMC counting and viability assessments, with offset level 2 closely matching the high performance of the LUNA-FX7™. However, it is essential to recognize that while the LUNA-III™ offers a cost-effective solution, using trypan blue (TB) staining can present challenges. TB staining is unable to differentiate PBMCs from contaminants like red blood cells and debris, potentially leading to overestimated cell counts and inaccurate viability readings, as contaminants may be miscounted as dead cells. Therefore, users must consider these limitations when working with PBMC samples, especially when higher accuracy is required in viability measurements.

https://www.wisbiomed.com/content/luna-iii-bright-field-automated-cell-counter-lb-l60001