Automated cell counting and viability measurement of Insect cells (SF9): Comparison of staining method

Staining Protocols

1. Mix:
• 10 μL TB, 0.4%
• 10 μL cell sample
2. Load 10 μL of stained cells.
3. Perform analysis using the LUNA-FX7™.
*Default protocol

Fluorescence staining

1. Mix:
• 2 μL pre-mixed dyes or 1μL per individual dye
• 18 μL cell sample
2. Load 10 μL of stained cells.
3. Perform analysis using the LUNA-FX7™.
*Note: SF9 cells may express varying fluorescent intensities based on dyes used. Please make adjustments as needed.

Dyes for Viability Measurement

We have selected four commonly used dyes for viability assessment: